Fees

Classical Cytogenetics

Classical chromosome study

Human non-tumor

$500


Non-human primate

$600


Human tumor

$1000


Rodent or other animal

$1000


Cell culture and chromosome study

Human non-tumor

$800


Non-human primate

$900


Human tumor

$1300


Rodent or other animal

$1300


Special stains, each (in addition to chromosome study)

$100


Chromosome harvesting, slidemaking, and banding without analysis

$300


AQA Testing

Cell line authentication
(DNA Fingerprinting by STR)

1 specimen

$145


2 specimen

$95 each


3+ specimen

$80 each


Mycoplasma testing
(two methods Staining and PCR)

1 specimen

$145


2 specimen

$95 each


3+ specimen

$80 each


Cell line authentication (Karyotyping)

Original cell line

$1000


later passages of the same cell line

$500


Species identification on G-banded metaphases without karyotyping

$300


Fluorescence In Situ Hybridization (FISH)

Chromosome or interphase nucleus preparation for in situ hybridization

$200


DNA probe acquisition and preparation (BAC purchase, culture, and DNA extraction)

$300


DNA probe labeling prior to hybridization (~1 µg)

$75


Control probe fee

$75


Interphase or chromosomal in situ hybridization, each probe

$150


Analysis fee for in situ hybridization

$300


Assessment of Chromosomal Instability

Aneuploidy Assessment: Counting chromosome number in 50 cells (solid stain)

$750


Frequency of PCC (100 cells)

$250


Breakage/Aberration Analysis (specimen plus control, solid stain; 200 cells each)

$2000


Miscellaneous Procedures

Primary cell culture

$450


EBV transformation

one blood specimen$150 each

$150 each


1two or more blood specimens

$125 each


Cell line distribution

$970


Cell Culture and Cytogenetics Facility
University of Pittsburgh
Department of Human Genetics
3060 Parran Hall Lab Annex-5C
130 De Soto Street
Pittsburgh PA, 15261

412-624-5358 (Laboratory)
412-624-5390 (Dr. Gollin's Office)