Catalog of Images from the UPCI Cell Culture and Cytogenetics Facility


Image 1: FISH

FHIT exons 5 (green) and 9/10 (red) hybridized to control lymphocyte metaphase. Images 1-3 are derived from the research of Susanne Gollin, Kay Huebner, and Carlo Croce, funded by NIH DE10513, CA39860, and CA21124.

Image 2: FISH

FHIT exons 5 and 9/10 hybridized to UPCI:SCC084 interphase nuclei. Note absence of one copy of each exon in this oral carcinoma cell line

Image 3: FISH

FHIT exons 5 and 9/10 hybridized to a UPCI:SCC056 interphase nucleus. Note absence of both copies of exon 5 and one copy of exons 9/10 in this oral carcinoma cell line.

Image 4: FISH

INT2 (red; 11q13) and D11Z1 (green; chromosome 11 centromere) probes hybridized to UPCI:SCC131 metaphase. Note amplification of INT2 in this oral carcinoma cell line. Probes from Oncor, Inc., Gaithersburg, MD. Results from the research of Susanne Gollin, funded by NIH DE10513.

Image 5:

Two representative G-banded metaphases from a mouse leukemia cell line.

Image 6: Abnormal NHP karyotype.

Abnormal NHP karyotype.

Image 7: Breast cancer cell line karyotype

Representative abnormal karyotype of a human breast cancer cell line studied by Dr. Jean Latime

Image 8: Paraffin section interphase FISH

Amplification of the cyclin D1 gene (CCND1) shown by fluorescence in situ hybridization (FISH) on a paraffin section of a head and neck squamous cell carcinoma..

Image 9: Metaphase FISH

Amplification of the cyclin D1 gene (CCND1) shown by fluorescence in situ hybridization on metaphase chromosomes from a head and neck squamous cell carcinoma cell line.

Image 10: Near-tetraploid murine karyotype.

Near-tetraploid murine chromosome complement.

Image 11: VNTR FISH

Identification of normal and aberrant chromosomes 15 in a CHO hybrid metaphase cell studied by Dr. Robert Nicholls.

Cell Culture and Cytogenetics Facility
University of Pittsburgh
Department of Human Genetics
3060 Parran Hall Lab Annex-5C
130 De Soto Street
Pittsburgh PA, 15261

412-624-5358 (Laboratory)
412-624-5390 (Dr. Gollin's Office)