Recent Cell Culture and Cytogenetics Facility Publications

  1. Brown DD, Dabbs DJ, Lee AV, McGuire KP, Ahrendt GM, Bhargava R, Davidson NE, Brufsky AM, Johnson RR, Oesterreich S, McAuliffe PF. Developing in vitro models of human ductal carcinoma in situ from primary tissue explants. Breast Cancer Res Treat 2015;153(2):311-321. doi: 10.1007/s10549-015-3551-8. PMID: 26283301.

  2. Li Y, Arao Y, Hall JM, Burkett S, Liu L, Gerrish K, Cavailles V, Korach KS. Research Resource: STR DNA profile and gene expression comparisons of human BG-1 cells and a BG-1/MCF-7 clonal variant. Mol Endocrinol 2014; 28(12):2072-2081. doi: 10.1210/me.2014-1229. PMID: 25321415.

  3. Parikh RA, Appleman LJ, Bauman JE, Sankunny M, Lewis DW, Vlad A, Gollin SM. Upregulation of the ATR-CHEK1 pathway in head and neck squamous cell carcinomas. Genes, Chromosomes, & Cancer 2014; 53(1):25-37. doi: 10.1002/gcc.22115.

  4. Sankunny M, Parikh RA, Lewis DW, Gooding WE, Saunders WS, Gollin SM. Targeted inhibition of ATR or CHEK1 reverses radioresistance in oral squamous cell carcinoma cells with distal chromosome arm 11q loss. Genes, Chromosomes, & Cancer 2014; 53(2):129-143. doi: 10.1002/gcc.22125.

  5. Lavasani M, Pollett JB, Usas A, Thompson SD, Pollett AF, Huard J. The microenvironment-specific transformation of adult stem cells models malignant triton tumors. PLoS One 2013; 8(12):e82173. doi: 10.1371/journal.pone.0082173. PMID: 24349213.

  6. Duuvuri U, Shiwarski DJ, Xiao D, Bertrand C, Huang X, Edinger RS, Rock J, Harfe BD, Henson BJ, Kunzelmann K, Schreiber R, Seethala RS, Egloff AM, Chen Z, Lui VW, Grandis JR, Gollin SM. TMEM16A induces MAPK and contributes directly to tumorigenesis and cancer progression. Cancer Res 2012;72(13):3270-3281. Epub 2012 May 7; PMID: 22564524.

  7. Monaghan SA, Dai L, Mapara MY, Normolle DP, Gollin SM, Lentzsch S. Longitudinal bone marrow evaluations for myelodysplasia in myeloma patients before and after treatment with lenalidomide. Leukemia & Lymphoma 2013; doi:10.3109/10428194.2012.755177. Posted online 10 December 2012. PMID: 23216269.

  8. Li Y, Feng H, Gu H, Lewis DW, Yuan Y, Zhang L, Yu H, Zhang P, Cheng H, Miao W, Yuan W, Cheng S-Y, Gollin SM, Cheng T. The p53-PUMA axis suppresses iPSC generation. Nature Commun 2013; 4:2174. doi: 10.1038/ncomms3174. PMID: 23873265.

  9. Wu Q, Sahasrabudhe RM, Luo LZ, Lewis DW, Gollin SM, Saunders WS. Deficiency in myosin light-chain phosphorylation causes cytokinesis failure and multipolarity in cancer cells. Oncogene 2010; 29(29):4183-4193. PMCID: PMC2911497.

  10. Vlasova II, Feng WH, Goff JP, Giorgianni A, Do D, Gollin SM, Lewis DW, Kagan VE, Yalowich JC. Myeloperoxidase-dependent oxidation of Etoposide in human myeloid progenitor CD34+ cells. Mol Pharmacol 2011; 79(3):479-87. Epub 2010 Nov 19. PMID: 21097707.

  11. Dufresne SD, Felgar RE, Sargent RL, Surti U, Gollin SM, McPhail ED, Cook JR, Swerdlow SH. Defining the borders of splenic marginal zone lymphoma: A multiparameter study. Human Pathol 2010; 41(4):540-551. PMID: 20004934. NIHMS #192543.

  12. Berman DM, Willman MA, Han D, Kleiner G, Kenyon NM, Cabrera O, Karl JA, Wiseman RW, O'Connor DH, Bartholomew AM, Kenyon NS. Mesenchymal stem cells enhance allogeneic islet engraftment in nonhuman primates. Diabetes 2010; 59(10):2558-2568. doi: 10.2337/db10-0136. PMID: 20622174.

  13. Uebing-Czipura AU, Dawson HD, Rutherford MS, Scherba G. Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb. Anim Biotechnol 2009; 20(4):186-215. doi: 10.1080/10495390903139950. PMID:19937495.

  14. Martin CL, Reshmi SC, Ried T, Gottberg W, Wilson JW, Reddy JK, Khanna P, Johnson JT, Myers EN, Gollin SM: Chromosomal imbalances in oral squamous cell carcinoma. Examination of 31 cell lines and review of the literature. Oral Oncol 2008; 44(4):369-82. PMCID: PMC2362065. (Dr. Thomas Ried, Head Cancer Genomics Section, Center for Cancer Research, National Cancer Institute, Bethesda, MD).

  15. Sargent RL, Cook JR, Aguilera NI, Surti U, Abbondanzo SL, Gollin SM, Swerdlow SH. Fluorescence immunophenotypic and interphase cytogenetic characterization of nodal lymphoplasmacytic lymphoma. Am J Surg Pathol 2008; 32(11):1643-53. (Dr. Cook is from the Case Western/Cleveland Clinic Cancer Center).

  16. Gaballah K, Costea DE, Hills A, Gollin SM, Harrison P, Partridge M. Tissue engineering of oral dysplasia. J Pathol 2008; 215(3):280-9. PMID: 18484684. (These collaborators are from the Depts. of Oncology and Oral and Maxillofacial Surgery, Kings College, London, UK).

  17. Odoux C, Fohrer H, Hoppo T, Guzik L, Stolz DB, Lewis DW, Gollin SM, Gamblin TC, Geller DA, Lagasse E. A stochastic model for cancer stem cell origin in metastatic colon cancer. Cancer Res. 2008; 68(17):6932-41. PMID: 18757407.

  18. Ragin CCR, Taioli E, Weissfeld JL, White JS, Rossie KM, Mudugno F, Gollin SM: 11q13 amplification, p16 and HPV in head and neck squamous cell carcinoma. Brit J Cancer 2006; 95:1432-1438.

  19. Reshmi SC, Huang X, Schoppy DW, Black RC, Saunders WS, Smith DI, Gollin SM: The relationship between FRA11F and 11q13 gene amplification in oral cancer. Genes, Chromosomes & Cancer 2007; 46:143-154.

  20. Reshmi SC, Roychaudhury S, Yu Z, Feingold E, Potter D, Saunders WS, Gollin SM: Inverted duplication pattern in anaphase bridges confirms the breakage-fusion-bridge (BFB) cycle model for 11q13 amplification. Cytogenetic and Genome Res 2007; 116:46-52.

  21. Wu G, Guo Z, Chatterjee A, Huang X, Rubin E, Wu F, Mambo E, Chang X, Osada M, Sook Kim M, Moon C, Califano JA, Ratovitski EA, Gollin SM, Sukumar S, Sidransky D, Trink B.: Overexpression of glycosylphosphatidylinositol (GPI) transamidase subunits phosphatidylinositol glycan class T and/or GPI anchor attachment 1 induces tumorigenesis and contributes to invasion in human breast cancer. Cancer Res 2006; 66:9829-9836. (Faculty from the Johns Hopkins Cancer Center utilized the UPCI Cytogenetics Facility for studies in this manuscript.)

  22. Parikh RA, White JS, Huang X, Schoppy DW, Baysal BE, Baskaran R, Bakkenist CJ, Saunders WS, Gollin SM: Loss of distal 11q is associated with DNA repair deficiency and reduced sensitivity to ionizing radiation in head and neck squamous cell carcinoma. Genes, Chromosomes Cancer 2007; 46(8):761-765.

  23. Soma LA, Gollin SM, Remstein ED, Ketterling RP, Flynn HC, Rajasenan KK, Swerdlow SH: Splenic small B-cell lymphoma with IGH/BCL3 translocation. Human Pathology 2006; 37(2):218-230.

  24. Wittschieben JP, Reshmi SC, Gollin SM, Wood RD: Loss of DNA polymerase zeta causes chromosomal instability in mammalian cells. Cancer Res 2006; 66:134-142.

  25. Shah US, Dhir R, Gollin SM, Chandran UR, Lewis D, Acquafondata M, Pflug BR: Fatty acid synthase gene overexpression and copy number gain in prostate adenocarcinoma. Human Pathology 2006; 37(4):401-409.

  26. Hsu L-C, Huang X, Seasholtz S, Potter DM, Gollin SM: Gene amplification and overexpression of protein phosphatase 1α in oral squamous cell carcinoma cell lines. Oncogene 2006; 25(40):5517-5526.

  27. Huang X, Godfrey TE, Gooding WE, McCarty KS, Gollin SM: Comprehensive genome and transcriptome analysis of the 11q13 amplicon in human oral cancer and synteny to the 7F5 amplicon in murine oral carcinoma. Genes, Chromosomes & Cancer 2006; 45:1058-1069.

  28. Reshmi SC, Gollin SM: Chromosomal instability in oral cancer cells. J. Dental Res 2005; 84:107-117.

  29. Wu G, Mambo E, Guo Z, Hu S, Huang X, Gollin SM, Trink B, Ladenson PW, Sidransky D, Xing M: Uncommon mutation but common amplifications of the PIK3CA gene in thyroid tumors. J Clin Endocrinol Metab 2005; 90:4688-4693. (Faculty from the Johns Hopkins Cancer Center utilized the UPCI Cytogenetics Facility for studies in this manuscript.)

  30. Cook JR, Aguilera NI, Reshmi S, Huang X, Yu Z, Gollin SM, Abbondanzo SL, Swerdlow SH: Deletion 6q is not a characteristic marker of nodal lymphoplasmacytic lymphoma. Cancer Genet Cytogenet. 2005; 162:85-88.

  31. Wu G, Xing M, Mambo E, Huang X, Liu J, Guo Z, Chatterjee A, Goldenberg D, Gollin SM, Sukumar S, Trink B, Sidransky D: Somatic mutation and gain of copy number of PIK3CA in human breast cancer. Breast Cancer Res 2005; 7:R609-616. PMCID: PMC1242128. (Faculty from the Johns Hopkins Cancer Center utilized the UPCI Cytogenetics Facility for studies in this manuscript.)

  32. Rose Ragin CC, Reshmi SC, Gollin SM: Mapping and analysis of HPV16 integration sites in a head and neck cancer cell line. Int J Cancer 2004; 110:701-709.

  33. Hewitt C, Wilson P, McGlinn E, MacFarlane G, Papageorgiou A, Woodwards RTM, Sloan P, Gollin SM, Patterson I, Parkinson KK, Read AP, Thakker N: DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma. Cancer Lett 2004; 209:207-213. (These investigators from the UK examined UPCI head & neck cancer cell lines on which cytogenetics had been characterized in the UPCI Cytogenetics Facility).

  34. Cook JR, Aguilera I, Reshmi-Skarja S, Huang X, Yu Z, Gollin SM, Abbondanzo SL, Swerdlow SH: Lack of PAX5 rearrangements in lymphoplasmacytic lymphomas: Reassessing the reported association with t(9;14). Hum Pathol 2004; 35:447-454.

  35. Reshmi SC, Saunders WS, Kudla DM, Rose Ragin CC, Gollin SM: Chromosomal instability and marker chromosome evolution in oral squamous cell carcinoma. Genes Chromosomes & Cancer 2004; 41:38-46.

  36. Luo LZ, Werner KM, Gollin SM, Saunders WS: Cigarette smoke induces anaphase bridges and genomic imbalances in normal cells. Mut Res 2004; 554:375-385.

Cell Culture and Cytogenetics Facility
University of Pittsburgh
Department of Human Genetics
3060 Parran Hall Lab Annex-5C
130 De Soto Street
Pittsburgh PA, 15261

412-624-5358 (Laboratory)
412-624-5390 (Dr. Gollin's Office)